Transgenic Core Facility
Transgenic Core Facility of CAF will be providing services for cryopreservation, recovery, in vitro fertilization, and rederivation of unique mice lines as well as for the generation of novel mice models.
Our services:
Cryopreservation and IVF services
- Mouse Sperm Cryopreservation
- Mouse Embryo Cryopreservation
- Mouse In Vitro Fertilization(IVF)
- Mouse Strain Rederivation from frozen stock
- Mouse Strain Rederivation to achieve pathogen-free status
- Rescue breeding Via IVF
- Colony expansion via IVF
Transgenics
- Generation of Transgenic Mice by DNA Microinjection (Traditional)
CRISPR/Cas9 Genome Editing and genotyping
- Guide RNA Testing in Mouse Zygotes
- Design and generation of DNA targeting constructs
- Generation of Knockout Mice Using Pronuclear microinjection / Electroportation
- Generation of small Knock-in Mice Using Single-Stranded Oligodeoxynucleotides (ssODNs)
- Generation of large Knock-in Mice Using Double-Stranded DNA (dsDNA) via 2 cell microinjection
- Generation of CRISPR mediated Conditional Knockout
- CRISPR-assisted ROSA26 Targeting with Double-stranded DNA (dsDNA) in Mouse Zygotes
- Founder and N1 Animal PCR Genotyping
- Founder and N1 Animal Sanger Sequencing
- Targeted Analysis of Off-Target Mutagenesis
Customized Services
You may contact the CAF Transgenic Core Facility at tg.caf@iisc.ac.in or saumyamathew@iisc.ac.in to discuss on projects.
Contact:
Dr. Saumya Mary Mathew
Transgenic Facility Manager
Central Animal Facility
Indian Institute of Science
Bangalore 560 012
INDIA
Phone : 91-80-22932734, 22932457
Email: tg.caf@iisc.ac.in